Decolorizing an anthraquinone dye byPhlebia brevispora: Intra-species characterization
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Purification and Characterization of a Novel Laccase from Cerrena sp. HYB07 with Dye Decolorizing Ability
Laccases (EC 1.10.3.2) are a class of multi-copper oxidases with important industrial values. A basidiomycete strain Cerrena sp. HYB07 with high laccase yield was identified. After cultivation in the shaking flask for 4 days, a maximal activity of 210.8 U mL(-1) was attained. A 58.6-kDa laccase (LacA) with 7.2% carbohydrate and a specific activity of 1952.4 U mg(-1) was purified. 2,2'-Azino-bis...
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Results Materials and Methods Four commercial dyes were used — Remazol® Black BA, Levafix® Blue Ca, Levafix® Yellow CA and Levafix® Red CA (Dystar). A standard anthraquinone dye, Remazol Brilliant Blue R (RBBR, Sigma) was also used. Dye stock solutions were added to solid or liquid decolorization media, up to 200 mg.L-1 final concentration. Rose Bengal Agar and Potato Dextrose Agar (both from D...
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ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2016
ISSN: 1618-0240
DOI: 10.1002/elsc.201600059